Wednesday, February 18, 2015

The Best Kit For Solubility Screening

By Beryl Dalton


Protein solubility kits are designed to help you in identifying the formulation that will protect your target proteins from aggregation. Solubility screening kits are also used to solubilize aggregated protein samples. The results you get from your tests will largely depend on the choice you make on the kit.

Some of the uses for the kit include quick aggregation behavior based on PH, salt and the presence of additives. It also assesses numerous conditions in a label free and parallel form. The processes require simple mixing and spinning without the use of centrifuge. This simplifies your tasks and allows you to get desired results in whichever condition.

The results obtained using the screening kits enable you to rescue reversibly aggregated protein samples. Your proteins will also not aggregate under stress. This makes them available for different tests and functions. You are provided with a dashboard with trends that make it easy to interpret results from the tests. The dashboard makes it easier to relate the results and make inferences.

The process of protein aggregation is undesirable because it hinders certain functions. Some of the conditions that lead to aggregation include addition of ligands, protein binding partners and elevated temperatures. Prolonged storage and vigorous stirring also cause aggregation. When the right decision on salt, PH and stabilizing additives is made, aggregation can be avoided.

The screening kit provides different buffers with PH ranging from three to ten as well as solubility enhancers. These enhancers include amino acids, polyols, salts, sugars and reducing agents, among others. This will help you determine the conditions under which your protein sample is protected from aggregation or the appropriate conditions for de-aggregation.

A single label free experiment can test more than ninety formulations. The other six experiments are used as positive and negative control tests. Soluble proteins will go through filters while the aggregated ones will not. This is a feature that is under examination during the filtration process.

Protein solubilization tests give the conditions under which your proteins will not aggregate. From such tests, it is observed that filtration processes yield proteins when they are in soluble form. They are available in aggregated form if the test does not show any traces of proteins.

The choice of conditions is determined by the results of screening. Since you can identify the conditions that facilitate the right behavior and the proteins in question, you can make an informed decision. It is used to confirm if the target substances are right for the expected results.

The benefits of using screening kits over other testing methods are numerous. Results obtained are clear and easy to interpret based on the information available in the field. The results leave no room for doubt. It takes as little as one hour to get your results. These results are certain and not based on trial and error.

The approach substantially reduces protein consumption during experiments. Because of this reduction, it is easy to match the target proteins with the assay. A detailed report is received allowing you to make many more inferences and conclusions. The kits do not restrict the assay that may be used during testing.




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